Protocol for a kit elisa example1

 

Preface

The template is the core of BioDataSheet, composing a good template means save days or months in the protocol and datasheet procedures.
The main concepts to keep in mind are:
  1. Template must reflect the general architecture of future documents
  2. Study your document(s) and find all parts in common, including all possible variables

Please note that variable values are not limited in dimensions and number, so you can consider to compose a big part of text as a variables. A good example considering a protocol for a kit elisa could be the BIBLIOGRAPHY part:
Bibliography can be stored in the BioDataSheet library, many times bibliography for the same molecule is the same, in the template chapter Bibliography, the only content become [Bibliography] variable and when I’ve to declare this value, the value can be copied and paste from the library. Easy, fast, no mistake.

Please note that number included in [ ] before a variable name, are system number that Biodatasheet needs to query and substitute the right values document by document.

  1. if you change the number and text inside [ ] you can have unexpected results
  2. if you insert the exact value expressed from drop down menu All variables, you can use an external editor to prepare chapters in Biodatasheet; simply copy and paste your text with variable names inside the right chapter and test the result.
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Chapter In Biodatasheet

(introduction)

 

The [2 - Product Name] is an E.L.I.S.A. for quantitative detection of [33 - Species] [32 - Molecule] in [34 - WhereKitWork] . The [2 - Product Name] is for research use only. Not for use in diagnostic or therapeutic procedures.

 

Variables Kit1

 

Variables

Declaration

[2 - Product Name] 

sCD137 (4-1BB) ELISA

[33 - Species]

human

[32 - Molecule]

CD137 (4-1BB)

[34 - WhereKitWork]

cell culture supernatants, human serum, plasma or other body fluids

 

Variables Kit2

 

Variables

Declaration

[2 - Product Name] 

sCD44var(5) ELISA

[33 - Species]

human

 [32 - Molecule]

soluble sCD44var(v5)

[34 - WhereKitWork]

cell culture supernatants, human serum, plasmas, amniotic fluid, or other body fluids

 

Result Kit021

 

The sCD137 (4-1BB) ELISA is an E.L.I.S.A. for quantitative detection of human soluble CD137 (4-1BB) in cell culture supernatants, human serum, plasma or other body fluids. The sCD137 (4-1BB) ELISA is for research use only. Not for use in diagnostic or therapeutic procedures.

 

Result Kit02

 

The sCD44var(5) ELISA is an E.L.I.S.A. for the quantitative detection of human soluble sCD44var(v5) levels in cell culture supernatants, human serum, plasmas, amniotic fluid, or other body fluids. The sCD44var(v5) ELISA is for research use only. Not for use in diagnostic or therapeutic procedures.

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TIPS and Tricks

 

What must be clear is that in the template study, some part of existing protocol/datasheet should be adapted to the Biodatasheet feature to foresee each possible value to insert in the variable.

In the final document you can add to a specific chapter (reagent provided for example) new lines of text not present in the template. The result is the template content plus the added text. Note that in this case when you change the template, is not possible change also what added, so there are two option: block the Document Chapter, template can not substitute the content, but smart is: create two chapter, one filled by template and one, empty in the template, filled by user and blocked to the template. In this case I can maintain both feature.

 
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